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General Topics => Political Discussion => Topic started by: aletheuo137 on February 28, 2022, 01:31:43 PM

Title: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Hu
Post by: aletheuo137 on February 28, 2022, 01:31:43 PM
https://www.mdpi.com/1467-3045/44/3/73/htm

Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure.

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Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: changemyoil66 on February 28, 2022, 01:43:01 PM
If only someone told the people that the MRNA tech was never approved for use on humans after the animal trials...................
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: aletheuo137 on February 28, 2022, 01:49:51 PM
If only someone told the people that the MRNA tech was never approved for use on humans after the animal trials...................
Tinfoil moment

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Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: eyeeatingfish on February 28, 2022, 08:10:06 PM
https://www.mdpi.com/1467-3045/44/3/73/htm

Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure.

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I'll admit I am not medical expert here, what is the takeaway of this in laymen terms?
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: macsak on February 28, 2022, 08:31:52 PM
I'll admit I am not medical expert here, what is the takeaway of this in laymen terms?

many people, both the creators of the vaccines, scientists, scientists-at-heart, and pro-vaxx influencers INSISTED that the mRNA in the vaccines could NEVER change your DNA
this study showed that in vitro (test tubes) that it gets incorporated into human DNA within a few hours...
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: aletheuo137 on March 01, 2022, 06:07:37 AM
many people, both the creators of the vaccines, scientists, scientists-at-heart, and pro-vaxx influencers INSISTED that the mRNA in the vaccines could NEVER change your DNA
this study showed that in vitro (test tubes) that it gets incorporated into human DNA within a few hours...
Thanks mac, well put!

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Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: macsak on March 01, 2022, 07:22:35 AM
#notarealdoctor


i also left out politicians and public health "experts"

Thanks mac, well put!

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Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: Rocky on March 01, 2022, 08:30:51 AM
I'll admit I am not medical expert here, what is the takeaway of this in laymen terms?

(https://i.imgur.com/bLVPSbGm.jpg)
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: changemyoil66 on March 01, 2022, 09:29:55 AM
I'll admit I am not medical expert here, what is the takeaway of this in laymen terms?

We know you're not a real doctor.
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: eyeeatingfish on March 09, 2022, 01:01:53 PM
many people, both the creators of the vaccines, scientists, scientists-at-heart, and pro-vaxx influencers INSISTED that the mRNA in the vaccines could NEVER change your DNA
this study showed that in vitro (test tubes) that it gets incorporated into human DNA within a few hours...

Found a good explanation of this study, it doesn't seem as bad as people are claiming it is. There are some issues with the study in terms of what it would mean in real life.

https://www.youtube.com/watch?v=cqfDXVzWbLM
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: macsak on March 09, 2022, 02:26:38 PM
please show me where i said it was bad or good..

Found a good explanation of this study, it doesn't seem as bad as people are claiming it is. There are some issues with the study in terms of what it would mean in real life.

https://www.youtube.com/watch?v=cqfDXVzWbLM
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: eyeeatingfish on March 14, 2022, 03:15:04 PM
please show me where i said it was bad or good..

I didn't say you said it was bad. I have seen a number of people bring up this story with negative implications.
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: macsak on March 14, 2022, 03:21:15 PM
I didn't say you said it was bad. I have seen a number of people bring up this story with negative implications.

the fact is that the pro-vaccine crowd all said it was impossible
it is possible...
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: eyeeatingfish on March 14, 2022, 03:26:49 PM
the fact is that the pro-vaccine crowd all said it was impossible
it is possible...

Did you see the circumstances that it occurred under in the study though?
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: macsak on March 14, 2022, 04:14:09 PM
Did you see the circumstances that it occurred under in the study though?

please show me where i said the circumstances in the study were reasonable or unreasonable
please show me where i said i didn't read the study
please show me where i said i didn't watch zdoggs video
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: eyeeatingfish on March 15, 2022, 02:23:48 PM
please show me where i said the circumstances in the study were reasonable or unreasonable
please show me where i said i didn't read the study
please show me where i said i didn't watch zdoggs video

I didn't say you said anything of that. I simply asked if you saw the video.
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: macsak on March 15, 2022, 03:21:17 PM
I didn't say you said anything of that. I simply asked if you saw the video.

focus

you did not "simply ask if i saw the video"...
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: eyeeatingfish on March 16, 2022, 09:34:28 AM
focus

you did not "simply ask if i saw the video"...

I said
"Did you see the circumstances that it occurred under in the study though?"

If you had not watched the video you would not have seen it, if you did then you would have. Obviously it would be irrelevant if you had seen the intro so I mentioned the section with relevance specifically.

BTW, you still didn't answer whether you saw it.
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: macsak on March 16, 2022, 12:25:34 PM
I said
"Did you see the circumstances that it occurred under in the study though?"

If you had not watched the video you would not have seen it, if you did then you would have. Obviously it would be irrelevant if you had seen the intro so I mentioned the section with relevance specifically.

BTW, you still didn't answer whether you saw it.

focus
there are multiple ways to see the circumstances without watching the zdogg video you posted

i could see the circumstances if i read the original study, which i did when it was released
i could see the circumstances if i watched the video, which i did when it was released
i could see the circumstances if i watched another video on the same study, which i did when it was released
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: eyeeatingfish on March 17, 2022, 02:00:31 PM
focus
there are multiple ways to see the circumstances without watching the zdogg video you posted

i could see the circumstances if i read the original study, which i did when it was released
i could see the circumstances if i watched the video, which i did when it was released
i could see the circumstances if i watched another video on the same study, which i did when it was released

That's good. I am glad you are aware of the circumstances.
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: macsak on March 17, 2022, 02:22:36 PM
That's good. I am glad you are aware of the circumstances.

thanks for trying to mansplain it to me so many times

i am glad you approve
makes my life whole again...
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: aletheuo137 on March 17, 2022, 06:57:29 PM
thanks for trying to mansplain it to me so many times

i am glad you approve
makes my life whole again...
Mansplaining (a blend word of man and the informal form splaining of the gerund explaining) is a pejorative term meaning (of a man) "to comment on or explain something to a woman in a condescending, overconfident, and often inaccurate or oversimplified manner"

Had to look that one up!

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Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: hvybarrels on March 17, 2022, 07:06:50 PM
Step 1: That's not true and you're a crazy person peddling misinformation who needs to be immediately cancelled.

Step 2: Well okay it is true but so what? It's not a big deal anyway. Chill out dude.

Step 3: We need to start another war before everyone figures out it's even worse than the conspiracy theorists imagined.
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: MauiAxis on March 19, 2022, 07:14:19 AM
Or maybe not?

https://healthfeedback.org/claimreview/study-lund-university-didnt-show-covid-19-mrna-vaccines-change-dna-epoch-times/
Title: Re: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro i
Post by: hvybarrels on March 19, 2022, 12:27:10 PM
Or maybe not?

https://healthfeedback.org/claimreview/study-lund-university-didnt-show-covid-19-mrna-vaccines-change-dna-epoch-times/

Oooh a fact checking website that Facebook uses to censor people.

looks like the science is settled, folks


(https://media.giphy.com/media/Rhhr8D5mKSX7O/giphy.gif)