Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Hu (Read 5916 times)

aletheuo137

https://www.mdpi.com/1467-3045/44/3/73/htm

Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure.

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changemyoil66

If only someone told the people that the MRNA tech was never approved for use on humans after the animal trials...................

aletheuo137

If only someone told the people that the MRNA tech was never approved for use on humans after the animal trials...................
Tinfoil moment

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eyeeatingfish

https://www.mdpi.com/1467-3045/44/3/73/htm

Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure.

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I'll admit I am not medical expert here, what is the takeaway of this in laymen terms?

macsak

I'll admit I am not medical expert here, what is the takeaway of this in laymen terms?

many people, both the creators of the vaccines, scientists, scientists-at-heart, and pro-vaxx influencers INSISTED that the mRNA in the vaccines could NEVER change your DNA
this study showed that in vitro (test tubes) that it gets incorporated into human DNA within a few hours...

aletheuo137

many people, both the creators of the vaccines, scientists, scientists-at-heart, and pro-vaxx influencers INSISTED that the mRNA in the vaccines could NEVER change your DNA
this study showed that in vitro (test tubes) that it gets incorporated into human DNA within a few hours...
Thanks mac, well put!

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macsak

#notarealdoctor


i also left out politicians and public health "experts"

Thanks mac, well put!

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Rocky

I'll admit I am not medical expert here, what is the takeaway of this in laymen terms?

“I ask you to judge me by the enemies I have made.”
                                                           Franklin D. Roosevelt

changemyoil66

I'll admit I am not medical expert here, what is the takeaway of this in laymen terms?

We know you're not a real doctor.

eyeeatingfish

many people, both the creators of the vaccines, scientists, scientists-at-heart, and pro-vaxx influencers INSISTED that the mRNA in the vaccines could NEVER change your DNA
this study showed that in vitro (test tubes) that it gets incorporated into human DNA within a few hours...

Found a good explanation of this study, it doesn't seem as bad as people are claiming it is. There are some issues with the study in terms of what it would mean in real life.

macsak

please show me where i said it was bad or good..

Found a good explanation of this study, it doesn't seem as bad as people are claiming it is. There are some issues with the study in terms of what it would mean in real life.


eyeeatingfish

please show me where i said it was bad or good..

I didn't say you said it was bad. I have seen a number of people bring up this story with negative implications.

macsak

I didn't say you said it was bad. I have seen a number of people bring up this story with negative implications.

the fact is that the pro-vaccine crowd all said it was impossible
it is possible...

eyeeatingfish

the fact is that the pro-vaccine crowd all said it was impossible
it is possible...

Did you see the circumstances that it occurred under in the study though?

macsak

Did you see the circumstances that it occurred under in the study though?

please show me where i said the circumstances in the study were reasonable or unreasonable
please show me where i said i didn't read the study
please show me where i said i didn't watch zdoggs video

eyeeatingfish

please show me where i said the circumstances in the study were reasonable or unreasonable
please show me where i said i didn't read the study
please show me where i said i didn't watch zdoggs video

I didn't say you said anything of that. I simply asked if you saw the video.

macsak

I didn't say you said anything of that. I simply asked if you saw the video.

focus

you did not "simply ask if i saw the video"...

eyeeatingfish

focus

you did not "simply ask if i saw the video"...

I said
"Did you see the circumstances that it occurred under in the study though?"

If you had not watched the video you would not have seen it, if you did then you would have. Obviously it would be irrelevant if you had seen the intro so I mentioned the section with relevance specifically.

BTW, you still didn't answer whether you saw it.

macsak

I said
"Did you see the circumstances that it occurred under in the study though?"

If you had not watched the video you would not have seen it, if you did then you would have. Obviously it would be irrelevant if you had seen the intro so I mentioned the section with relevance specifically.

BTW, you still didn't answer whether you saw it.

focus
there are multiple ways to see the circumstances without watching the zdogg video you posted

i could see the circumstances if i read the original study, which i did when it was released
i could see the circumstances if i watched the video, which i did when it was released
i could see the circumstances if i watched another video on the same study, which i did when it was released

eyeeatingfish

focus
there are multiple ways to see the circumstances without watching the zdogg video you posted

i could see the circumstances if i read the original study, which i did when it was released
i could see the circumstances if i watched the video, which i did when it was released
i could see the circumstances if i watched another video on the same study, which i did when it was released

That's good. I am glad you are aware of the circumstances.